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cfs1 filter slider  (Thorlabs)

 
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    Structured Review

    Thorlabs cfs1 filter slider
    ( A ) Schematic of single-camera <t>microscope</t> system configurations and ( B ) spectra of deposited dye absorbance (amplitude normalized). Shading indicates the spectral region in which the photopic visual response (relative luminosity factors) is >1% of the maximum near 554 nm . HTX = hematoxylin; AMC = tyramide-derivatized 7-amino-4-methylcoumarin-3-acetate; NMC = azide of 7-(methylamino)coumarin-3-acetate; sCy7 or Cy7 = azide of sulfo-Cyanine 7; ir870 = azide of novel chloro-sCy7 derivative; HTX = hematoxylin; DAB = 3,3′-diaminobenzidine.
    Cfs1 Filter Slider, supplied by Thorlabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfs1+filter+slider/pmc11764411-69-57-50?v=Thorlabs
    Average 90 stars, based on 1 article reviews
    cfs1 filter slider - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens"

    Article Title: Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens

    Journal: Diagnostics

    doi: 10.3390/diagnostics15020164

    ( A ) Schematic of single-camera microscope system configurations and ( B ) spectra of deposited dye absorbance (amplitude normalized). Shading indicates the spectral region in which the photopic visual response (relative luminosity factors) is >1% of the maximum near 554 nm . HTX = hematoxylin; AMC = tyramide-derivatized 7-amino-4-methylcoumarin-3-acetate; NMC = azide of 7-(methylamino)coumarin-3-acetate; sCy7 or Cy7 = azide of sulfo-Cyanine 7; ir870 = azide of novel chloro-sCy7 derivative; HTX = hematoxylin; DAB = 3,3′-diaminobenzidine.
    Figure Legend Snippet: ( A ) Schematic of single-camera microscope system configurations and ( B ) spectra of deposited dye absorbance (amplitude normalized). Shading indicates the spectral region in which the photopic visual response (relative luminosity factors) is >1% of the maximum near 554 nm . HTX = hematoxylin; AMC = tyramide-derivatized 7-amino-4-methylcoumarin-3-acetate; NMC = azide of 7-(methylamino)coumarin-3-acetate; sCy7 or Cy7 = azide of sulfo-Cyanine 7; ir870 = azide of novel chloro-sCy7 derivative; HTX = hematoxylin; DAB = 3,3′-diaminobenzidine.

    Techniques Used: Microscopy

    H&E + IHC specimen and light channel information. Specimens were stained by single or multiplex IHC for the indicated biomarkers and chromogens. The illumination channel for each chromogen is listed, and figure numbers in which images of each are presented. H&E and DAB plus hematoxylin stains were viewed and imaged using visible white light illumination from a tungsten microscope lamp through a 420–690 nm (> 85% transmission) filter. DAB was additionally viewed under 405 nm illumination. Additional filter and LED information, including sources, is listed in <xref ref-type= Supplementary Table S1 ." title="... using visible white light illumination from a tungsten microscope lamp through a 420–690 nm (> ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: H&E + IHC specimen and light channel information. Specimens were stained by single or multiplex IHC for the indicated biomarkers and chromogens. The illumination channel for each chromogen is listed, and figure numbers in which images of each are presented. H&E and DAB plus hematoxylin stains were viewed and imaged using visible white light illumination from a tungsten microscope lamp through a 420–690 nm (> 85% transmission) filter. DAB was additionally viewed under 405 nm illumination. Additional filter and LED information, including sources, is listed in Supplementary Table S1 .

    Techniques Used: Staining, Multiplex Assay, Microscopy, Transmission Assay, Biomarker Discovery, Immunohistochemistry

    H&E + invisible IHC images using the dual-camera microscope system ( A ) and single-camera microscope system ( B – D ). ( A ) Dual-camera microscope monitor screenshot simultaneously displaying visible H&E staining (left side, color camera video) and non-visible LAG3 sCy7 IHC staining (right side, monochrome camera video) of melanoma FFPE tissue. The specimen was simultaneously illuminated with white light from a tungsten microscope lamp through a broad white light filter (420–620 nm) and NIR light from a 770 nm LED. ( B , C ) Sequential view of H&E staining ( B ) and non-visible HER2 IHC using ir870 chromogen ( C ), on breast tumor FFPE tissue, using same color camera (IR-blocking filter removed). The specimen was illuminated with a tungsten microscope lamp while switching between the broad white light filter ( B ) and an 880 nm bandpass filter ( C ). ( D ) HER2 image displayed in grayscale (blue color plane of color image in part C ). Circles highlight two individual cells in images of each specimen. The color saturation of ( C ) was reduced to 60% to aid in viewing the printed blue image.
    Figure Legend Snippet: H&E + invisible IHC images using the dual-camera microscope system ( A ) and single-camera microscope system ( B – D ). ( A ) Dual-camera microscope monitor screenshot simultaneously displaying visible H&E staining (left side, color camera video) and non-visible LAG3 sCy7 IHC staining (right side, monochrome camera video) of melanoma FFPE tissue. The specimen was simultaneously illuminated with white light from a tungsten microscope lamp through a broad white light filter (420–620 nm) and NIR light from a 770 nm LED. ( B , C ) Sequential view of H&E staining ( B ) and non-visible HER2 IHC using ir870 chromogen ( C ), on breast tumor FFPE tissue, using same color camera (IR-blocking filter removed). The specimen was illuminated with a tungsten microscope lamp while switching between the broad white light filter ( B ) and an 880 nm bandpass filter ( C ). ( D ) HER2 image displayed in grayscale (blue color plane of color image in part C ). Circles highlight two individual cells in images of each specimen. The color saturation of ( C ) was reduced to 60% to aid in viewing the printed blue image.

    Techniques Used: Microscopy, Staining, Immunohistochemistry, Blocking Assay

    Hodgkin’s lymphoma FFPE specimen, 2-plex IHC (CD30 AMCA/CD15 sCy7) + H&E, imaged with color camera (IR-blocking filter removed). ( A ) H&E imaged under white light illumination (tungsten microscope lamp with IR-blocking filter removed plus 420–690 nm broadband filter). ( B ) CD30 IHC using AMC chromogen and illuminated with UV light (365 nm LED plus 375 nm single bandpass filter). ( C ) CD15 IHC using sCy7 chromogen with NIR illumination (tungsten lamp with IR-blocking filter removed plus 769 nm single bandpass filter). ( D ) Grayscale representation of color image B (CD30; blue image plane). ( E ) Grayscale representation of color image C (CD15; red image plane). Circles highlight three individual cells under different illumination. The color saturation of ( B ) was reduced to 60% to aid in viewing the printed blue image.
    Figure Legend Snippet: Hodgkin’s lymphoma FFPE specimen, 2-plex IHC (CD30 AMCA/CD15 sCy7) + H&E, imaged with color camera (IR-blocking filter removed). ( A ) H&E imaged under white light illumination (tungsten microscope lamp with IR-blocking filter removed plus 420–690 nm broadband filter). ( B ) CD30 IHC using AMC chromogen and illuminated with UV light (365 nm LED plus 375 nm single bandpass filter). ( C ) CD15 IHC using sCy7 chromogen with NIR illumination (tungsten lamp with IR-blocking filter removed plus 769 nm single bandpass filter). ( D ) Grayscale representation of color image B (CD30; blue image plane). ( E ) Grayscale representation of color image C (CD15; red image plane). Circles highlight three individual cells under different illumination. The color saturation of ( B ) was reduced to 60% to aid in viewing the printed blue image.

    Techniques Used: Blocking Assay, Microscopy



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    ( A ) Schematic of single-camera <t>microscope</t> system configurations and ( B ) spectra of deposited dye absorbance (amplitude normalized). Shading indicates the spectral region in which the photopic visual response (relative luminosity factors) is >1% of the maximum near 554 nm . HTX = hematoxylin; AMC = tyramide-derivatized 7-amino-4-methylcoumarin-3-acetate; NMC = azide of 7-(methylamino)coumarin-3-acetate; sCy7 or Cy7 = azide of sulfo-Cyanine 7; ir870 = azide of novel chloro-sCy7 derivative; HTX = hematoxylin; DAB = 3,3′-diaminobenzidine.
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    ( A ) Schematic of single-camera <t>microscope</t> system configurations and ( B ) spectra of deposited dye absorbance (amplitude normalized). Shading indicates the spectral region in which the photopic visual response (relative luminosity factors) is >1% of the maximum near 554 nm . HTX = hematoxylin; AMC = tyramide-derivatized 7-amino-4-methylcoumarin-3-acetate; NMC = azide of 7-(methylamino)coumarin-3-acetate; sCy7 or Cy7 = azide of sulfo-Cyanine 7; ir870 = azide of novel chloro-sCy7 derivative; HTX = hematoxylin; DAB = 3,3′-diaminobenzidine.
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    Image Search Results


    ( A ) Schematic of single-camera microscope system configurations and ( B ) spectra of deposited dye absorbance (amplitude normalized). Shading indicates the spectral region in which the photopic visual response (relative luminosity factors) is >1% of the maximum near 554 nm . HTX = hematoxylin; AMC = tyramide-derivatized 7-amino-4-methylcoumarin-3-acetate; NMC = azide of 7-(methylamino)coumarin-3-acetate; sCy7 or Cy7 = azide of sulfo-Cyanine 7; ir870 = azide of novel chloro-sCy7 derivative; HTX = hematoxylin; DAB = 3,3′-diaminobenzidine.

    Journal: Diagnostics

    Article Title: Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens

    doi: 10.3390/diagnostics15020164

    Figure Lengend Snippet: ( A ) Schematic of single-camera microscope system configurations and ( B ) spectra of deposited dye absorbance (amplitude normalized). Shading indicates the spectral region in which the photopic visual response (relative luminosity factors) is >1% of the maximum near 554 nm . HTX = hematoxylin; AMC = tyramide-derivatized 7-amino-4-methylcoumarin-3-acetate; NMC = azide of 7-(methylamino)coumarin-3-acetate; sCy7 or Cy7 = azide of sulfo-Cyanine 7; ir870 = azide of novel chloro-sCy7 derivative; HTX = hematoxylin; DAB = 3,3′-diaminobenzidine.

    Article Snippet: A 100 W tungsten-halogen microscope lamp (Olympus model U-LH100) was the source for broad-spectrum light, and the means to select different bands of light was either a Sutter Lambda 10-3 10-position filter wheel (Sutter Instruments, Novato, CA, USA) placed between the microscope lamp and the microscope illumination port, or a Thorlabs CFS1 filter slider placed between the microscope camera port and color camera. lists the different filters used for illuminating the various stains in work presented here.

    Techniques: Microscopy

    H&E + IHC specimen and light channel information. Specimens were stained by single or multiplex IHC for the indicated biomarkers and chromogens. The illumination channel for each chromogen is listed, and figure numbers in which images of each are presented. H&E and DAB plus hematoxylin stains were viewed and imaged using visible white light illumination from a tungsten microscope lamp through a 420–690 nm (> 85% transmission) filter. DAB was additionally viewed under 405 nm illumination. Additional filter and LED information, including sources, is listed in <xref ref-type= Supplementary Table S1 ." width="100%" height="100%">

    Journal: Diagnostics

    Article Title: Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens

    doi: 10.3390/diagnostics15020164

    Figure Lengend Snippet: H&E + IHC specimen and light channel information. Specimens were stained by single or multiplex IHC for the indicated biomarkers and chromogens. The illumination channel for each chromogen is listed, and figure numbers in which images of each are presented. H&E and DAB plus hematoxylin stains were viewed and imaged using visible white light illumination from a tungsten microscope lamp through a 420–690 nm (> 85% transmission) filter. DAB was additionally viewed under 405 nm illumination. Additional filter and LED information, including sources, is listed in Supplementary Table S1 .

    Article Snippet: A 100 W tungsten-halogen microscope lamp (Olympus model U-LH100) was the source for broad-spectrum light, and the means to select different bands of light was either a Sutter Lambda 10-3 10-position filter wheel (Sutter Instruments, Novato, CA, USA) placed between the microscope lamp and the microscope illumination port, or a Thorlabs CFS1 filter slider placed between the microscope camera port and color camera. lists the different filters used for illuminating the various stains in work presented here.

    Techniques: Staining, Multiplex Assay, Microscopy, Transmission Assay, Biomarker Discovery, Immunohistochemistry

    H&E + invisible IHC images using the dual-camera microscope system ( A ) and single-camera microscope system ( B – D ). ( A ) Dual-camera microscope monitor screenshot simultaneously displaying visible H&E staining (left side, color camera video) and non-visible LAG3 sCy7 IHC staining (right side, monochrome camera video) of melanoma FFPE tissue. The specimen was simultaneously illuminated with white light from a tungsten microscope lamp through a broad white light filter (420–620 nm) and NIR light from a 770 nm LED. ( B , C ) Sequential view of H&E staining ( B ) and non-visible HER2 IHC using ir870 chromogen ( C ), on breast tumor FFPE tissue, using same color camera (IR-blocking filter removed). The specimen was illuminated with a tungsten microscope lamp while switching between the broad white light filter ( B ) and an 880 nm bandpass filter ( C ). ( D ) HER2 image displayed in grayscale (blue color plane of color image in part C ). Circles highlight two individual cells in images of each specimen. The color saturation of ( C ) was reduced to 60% to aid in viewing the printed blue image.

    Journal: Diagnostics

    Article Title: Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens

    doi: 10.3390/diagnostics15020164

    Figure Lengend Snippet: H&E + invisible IHC images using the dual-camera microscope system ( A ) and single-camera microscope system ( B – D ). ( A ) Dual-camera microscope monitor screenshot simultaneously displaying visible H&E staining (left side, color camera video) and non-visible LAG3 sCy7 IHC staining (right side, monochrome camera video) of melanoma FFPE tissue. The specimen was simultaneously illuminated with white light from a tungsten microscope lamp through a broad white light filter (420–620 nm) and NIR light from a 770 nm LED. ( B , C ) Sequential view of H&E staining ( B ) and non-visible HER2 IHC using ir870 chromogen ( C ), on breast tumor FFPE tissue, using same color camera (IR-blocking filter removed). The specimen was illuminated with a tungsten microscope lamp while switching between the broad white light filter ( B ) and an 880 nm bandpass filter ( C ). ( D ) HER2 image displayed in grayscale (blue color plane of color image in part C ). Circles highlight two individual cells in images of each specimen. The color saturation of ( C ) was reduced to 60% to aid in viewing the printed blue image.

    Article Snippet: A 100 W tungsten-halogen microscope lamp (Olympus model U-LH100) was the source for broad-spectrum light, and the means to select different bands of light was either a Sutter Lambda 10-3 10-position filter wheel (Sutter Instruments, Novato, CA, USA) placed between the microscope lamp and the microscope illumination port, or a Thorlabs CFS1 filter slider placed between the microscope camera port and color camera. lists the different filters used for illuminating the various stains in work presented here.

    Techniques: Microscopy, Staining, Immunohistochemistry, Blocking Assay

    Hodgkin’s lymphoma FFPE specimen, 2-plex IHC (CD30 AMCA/CD15 sCy7) + H&E, imaged with color camera (IR-blocking filter removed). ( A ) H&E imaged under white light illumination (tungsten microscope lamp with IR-blocking filter removed plus 420–690 nm broadband filter). ( B ) CD30 IHC using AMC chromogen and illuminated with UV light (365 nm LED plus 375 nm single bandpass filter). ( C ) CD15 IHC using sCy7 chromogen with NIR illumination (tungsten lamp with IR-blocking filter removed plus 769 nm single bandpass filter). ( D ) Grayscale representation of color image B (CD30; blue image plane). ( E ) Grayscale representation of color image C (CD15; red image plane). Circles highlight three individual cells under different illumination. The color saturation of ( B ) was reduced to 60% to aid in viewing the printed blue image.

    Journal: Diagnostics

    Article Title: Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens

    doi: 10.3390/diagnostics15020164

    Figure Lengend Snippet: Hodgkin’s lymphoma FFPE specimen, 2-plex IHC (CD30 AMCA/CD15 sCy7) + H&E, imaged with color camera (IR-blocking filter removed). ( A ) H&E imaged under white light illumination (tungsten microscope lamp with IR-blocking filter removed plus 420–690 nm broadband filter). ( B ) CD30 IHC using AMC chromogen and illuminated with UV light (365 nm LED plus 375 nm single bandpass filter). ( C ) CD15 IHC using sCy7 chromogen with NIR illumination (tungsten lamp with IR-blocking filter removed plus 769 nm single bandpass filter). ( D ) Grayscale representation of color image B (CD30; blue image plane). ( E ) Grayscale representation of color image C (CD15; red image plane). Circles highlight three individual cells under different illumination. The color saturation of ( B ) was reduced to 60% to aid in viewing the printed blue image.

    Article Snippet: A 100 W tungsten-halogen microscope lamp (Olympus model U-LH100) was the source for broad-spectrum light, and the means to select different bands of light was either a Sutter Lambda 10-3 10-position filter wheel (Sutter Instruments, Novato, CA, USA) placed between the microscope lamp and the microscope illumination port, or a Thorlabs CFS1 filter slider placed between the microscope camera port and color camera. lists the different filters used for illuminating the various stains in work presented here.

    Techniques: Blocking Assay, Microscopy